Real Time PCR (qPCR)

96-wells block format - sample block is made of gold-coated silver to provide outstanding thermal conductivity, uniformity as well as fast
heating and cooling ramping rates - Sample volume: 5 – 100 μl (10 to 80 μl recommended) - Lid temperature: 30 – 110 °C - Temperature
gradient: 40°C (0.1°C steps) - Heating rate adjustment: min. 0.1 °C/s - Temperature uniformity: ±0.15 °C at 55 °C; ±0.25 °C at 72 °C; ±0.50 °C at
95 °C - Temperature range: 3 °C – 99 °C - Control accuracy: ± 0.1 °C - Temperature increments: min. 0.1 °C/cycle - Time increments: min. 1
s/cycle - Heated lid: Manual opening mechanism, automatic contact pressure - Noise level: 45 dB - Supported plastic products: 96-well micro
titer plates with optical film, 8-well strips 0.2 ml with optical lids 0.2 ml individual vessels with optical lids - Sensitivity: 1 nmol/l FAM at 30μl
sample volume in a 96-well PCR plate - Measuring time: 96-well plate (single measurement, 6 colors) approximately 6 s - Measuring range:
±130 000 (±17 bit) - Light source: Four high-intensity LEDs (blue, green, white, red) - Photo Multiplier (PMT) detector - Color filter modules for
all frequently used real-time PCR dyes: Blue channel: 465±15 nm Excitation / 524±12 nm Detection (FAM™, SYBR® Green, EvaGreen®) Green
channel: 510±15 nm Excitation) / 565±15 Detection nm (JOE™, HEX™, VIC®) Orange channel: 560±15 nm Excitation) / 610±15 Detection nm
(ROX™, Texas Red®) Red channel: 625±10 nm Excitation) / 680±15 Detection nm (Cy5®, Alexa Fluor® 647) NIR 1 channel: 625±10 nm
Excitation) / 710±20 Detection nm (Cy5.5®, Quasar® 705) - Analysis software: Absolute quantification, relative quantification, ΔΔCt method,
allelic discrimination, efficiency calculation, DNA melting curves, POS/NEG analysis in the end point - USB data connection Including : -Computer & Isotrans